Cord Blood Collection Protocol - Step 1 - Collect Cord Blood Sample

Instructions for Collection Prior to Expulsion of Placenta

1. After delivery of the infant and prior to expulsion of the placenta, double-clamp and cut the umbilical cord.
2. Sterilize the proposed needle insertion site of the cord with the antiseptic prep pad provided. The proposed needle insertion site should be just above the clamp that remains on the cord.
3. Insert the needle of the Collection Bag provided into the cord and collect as much sample as possible via gravity-flow, aiming to fill the bag (a minimum of 80 mL is required). The cord will appear empty and pale when collection is completed.
4. Tie two tight knots in the tubing as close to the needle as possible.
5. Gently rotate the bag several times to thoroughly mix the cord blood with the anticoagulant.

Instructions for Collection Following Expulsion of Placenta or After Cesarean Delivery

1. Double-clamp and cut the umbilical cord.
2. Place the placenta and cord in a sterile tray, bowl, or Chux pad with the fetal side down.
3. Place the placenta on an elevated surface if possible, and drape the umbilical cord out of the tray or Chux pad.
4. Sterilize the proposed needle insertion site of the cord with the antiseptic prep pad provided. The proposed needle insertion site should be just above the clamp that remains on the cord.
5. Insert the needle of the Collection Bag provided into the cord and collect as much sample as possible via gravity-flow, aiming to fill the bag (a minimum of 80 mL is required). The cord will appear empty and pale when collection is completed.
6. Tie two tight knots in the tubing as close to the needle as possible.
7. Gently rotate the bag several times to thoroughly mix the cord blood with the anticoagulant.

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